Coding
PETase

Part:BBa_K2984069:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


PETase CDS; Enzyme that catalyzes the first degradation step of PET


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 861
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 400
    Illegal PstI site found at 861
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 861
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 861
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is used to make our composite parts compatible for the iGEM registry. This part is not MoClo compatible because it does not have the MoClo overhangs.



Source

Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., ... & Oda, K. (2016). A bacterium that degrades and assimilates poly (ethylene terephthalate). Science, 351(6278), 1196-1199.

References